Whole Genome Amplification Method Obtaining a Stable Stock for Genome Research

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High-throughput genetic studies often require large quantities of DNA for a variety of analyses. We developed a whole genome amplification method, called SATAN, which is based on an adaptor-ligation mediated PCR of randomly fragmented DNAs. Loss of exon and artificial monoallelic amplification were demonstrated to be quite infrequent. Of 261 amplifiable microsatellites on various chromosomes, 259 (99.2 %) show a consistent pattern between the SATAN product and the original DNA. DNA array CGH analysis of 287 loci for measuring relative gene copy number demonstrated that a quite low bias was detected. Moreover, SATAN was validated on 100-1000 laser-captured cells from paraffin-embedded tissues. We thus concluded that SATAN provides a sufficient amount of genomic sequence for a variety of genetic analyses as well as for long-term storage for future work.