Cancer Proteomics for Biomarker Development

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Tadashi Kondo

Abstract
To develop biomarker by cancer proteomics, the proteomics system was established in the Proteome Bioinformatics Project at the National Cancer Center. The experimental system is characterized by the use of widely available instruments and reagents as well as its high-efficiency, high-accuracy, and high-throughput data. The detailed protocols established in the project were published with an aim of standardization of protocols. Using the established experimental system and collaboration with physicians in the National Cancer Center Hospital, the biomarker candidates were identified for personalized medicine. Parts of the obtained proteome results were opened as activities of the GeMDBJ Proteomics.

Reference
1. Tadashi Kondo and Sestuo Hirohashi, Application of highly sensitive fluorescent dyes (CyDye DIGE Fluor saturation dyes) to laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE) for cancer proteomics. Nat Protoc, 1, 2940-2956, 2006.
2. Tadashi Kondo, Tissue proteomics for cancer biomarker development - Laser microdissection and 2D-DIGE -, BMB report, 41, 625-634, 2008.
3. Masayo Yamada, Kiyonaga Fujii, Koji Koyama, Setsuo Hirohashi, Tadashi Kondo, The Proteomic Profile of Pancreatic Cancer Cell Lines Corresponding to Carcinogenesis and Metastasis. J Proteom Bioinform, 2, 1-18, 2009.