Development for early detection of colorectal cancer using fecal sample
Development for early detection of colorectal cancer using fecal samplesWe are developing and studying an early diagnosis method of colorectal cancer using exfoliated cancer cells in fecal sample. Colorectal cancer occurs from a colon mucosa and is always in contact with feces. For that reason, fecal sample of colorectal cancer patients contained exfoliated cancer cells, and we developed and reported a cell separation method using immuno-magnetic beads conjugated with anti-human EpCAM antibody. Following the cell isolation method, mutation analysis of cancer-specific genes such as APC, Kras, and p53 was reported using a sequencing method and SSCP method. We have reported expression analyses of cancer-specific genes (such as COX2, MYBL2, MMP7, and p53) and cancer-specific miRNA (such as miRNA miR-17-92 cluster, miR-21, and miR-135). To develop a new colorectal cancer screening method combined with fecal occult blood test (FOBT) and fecal miRNA test (FmiRT), we have reported that miRNA can be efficiently extracted from a residual sample of FOBT and FmiRT combined with FOBT may improve the sensitivity to detect colorectal cancer. Now, we are evaluating not only FmiRT but also highly sensitive fecal protein test for colorectal cancer screening.
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- Koga Y, Yasunaga M, Katayose S, Moriya Y, Akasu T, Fujita S, Yamamoto S, Baba H, Matsumura Y. Improved recovery of exfoliated colonocytes from feces using newly developed immunomagnetic beads. Gastroenterol Res Pract, 2008:605273. doi: 10.1155/2008/605273. [PubMed]
- Onouchi S, Matsushita H, Moriya Y, Akasu T, Fujita S, Yamamoto S, Hasegawa H, Kitagawa Y, Matsumura Y. New method for colorectal cancer diagnosis based on SSCP analysis of DNA from exfoliated colonocytes in naturally evacuated feces. Anticancer Res, 28:145-150, 2008 [PubMed]
- Koga Y, Yasunaga M, Moriya Y, Akasu T, Fujita S, Yamamoto S, Kozu T, Baba H, Matsumura Y. Detection of colorectal cancer cells from feces using quantitative real-time RT-PCR for colorectal cancer diagnosis. Cancer Sci, 99:1977-1983, 2008 [PubMed]
- Koga Y, Yasunaga M, Takahashi A, Kuroda J, Moriya Y, Akasu T, Fujita S, Yamamoto S, Baba H, Matsumura Y. MicroRNA expression profiling of exfoliated colonocytes isolated from feces for colorectal cancer screening. Cancer Prev Res (Phila), 3:1435-1442, 2010 [PubMed]
- Yamazaki N, Koga Y, Yamamoto S, Kakugawa Y, Otake Y, Hayashi R, Saito N, Matsumura Y. Application of the fecal micro RNA test to the residuum from the fecal occult blood test. Jpn J Clin Oncol, 43(7): 726-733, 2013.
- Koga Y, Yamazaki N, Yamamoto Y, Yamamoto S, Saito N, Kakugawa Y, Otake Y, Matsumoto M, Matsumura Y. Fecal miR-106a is a useful marker for colorectal cancer patients with false-negative results in immunochemical fecal occult blood test. Cancer Epidemiol Biomarker Prev, 22(10): 1844-1852, 2013.