Annual Report 2018
Central Animal Division
Toshio Imai, Mami Takahashi, Mie Naruse, Rikako Ishigamori, Angeline Teh Ping Ping, Naoaki Uchiya, Ruri Nakanishi, Yurika Shiotani, Makiko Saitou, Momo Nakanishi, Yoshinori Ikarashi, Yukino Machida, Masashi Yasuda, Noriyuki Kimura, Junichi Zukeyama, Hiroyuki Hayashida, Saori Kobayashi, Sakura Ueki, Ikuko Harada
Introduction
The important role of the Central Animal Division is health management of experimental animals and maintenance of animal experimentation facilities. Some researchers and technical staff also act for several support services, which are provided based on their biological skills, such as reproductive technologies for animal cleaning / embryo-sperm preservation, pathological analysis of mouse phenotypes, and establishment of expandable cells / xenograft transplantable models from clinical cancer tissues (PDX models).
The Team and What We Do
Animal models are essential not only for basic cancer research but also for translational research (TR) fields. We willingly contribute to establish useful animal models including genetically engineered mice (GEM) for basic cancer research, PDX models for TR and to analyze their molecular details.
Research activities
As for PDX models, genetic and/or phenotypic changes by their passages and characterizations of proliferative lesions originated from human lymphocytes have been evaluated. Our members have established PDX models mainly from colorectal, pancreas and uterine endometrial cancers.
Education
We contribute to organize the annual training sessions for researchers and technical experts on animal experiments. In addition, technical trainees are received for skill acquirement for animal experiments and mouse phenotype analysis.
Future prospects
Research approaches using immune deficient/severely immune-deficient mice have become increasingly important over the past few years, and microbiological controls of the animal experimentation facility should become more strictly controlled.
List of papers published in 2018
Journal
1. Oyama R, Takahashi M, Kito F, Sakumoto M, Takai Y, Shiozawa K, Qiao Z, Toki S, Tanzawa Y, Yoshida A, Kawai A, Kondo T. Establishment and characterization of patient-derived pleomorphic rhabdomyosarcoma models. Tiss Cult Res Commun, 38:1-12, 2019
2. Hori M, Mutoh M, Ishigamori R, Imai T, Takahashi M. Activated ductal proliferation induced by N-nitrosobis (2-oxopropyl)amine in fat-infiltrated pancreas of KK-A(y) mice. In Vivo, 32:499-505, 2018
3. Takahashi M, Hori M, Ishigamori R, Mutoh M, Imai T, Nakagama H. Fatty pancreas: A possible risk factor for pancreatic cancer in animals and humans. Cancer Sci, 109:3013-3023, 2018
4. Oyama R, Takahashi M, Kito F, Sakumoto M, Shiozawa K, Qiao Z, Yoshida A, Endo M, Kawai A, Kondo T. Establishment and characterization of patient-derived xenograft and its cell line of primary leiomyosarcoma of bone. In Vitro Cell Dev Biol Anim, 54:458- 467, 2018
5. Kito F, Oyama R, Sakumoto M, Takahashi M, Shiozawa K, Qiao Z, Sakamoto H, Hirose T, Setsu N, Yoshida A, Kawai A, Kondo T. Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line. In Vitro Cell Dev Biol Anim, 54:528-536, 2018
6. Oyama R, Kito F, Qiao Z, Sakumoto M, Noguchi R, Takahashi M, Toki S, Tanzawa Y, Yoshida A, Kawai A, Kondo T. Establishment of a novel patient-derived Ewing's sarcoma cell line, NCCES1-C1. In Vitro Cell Dev Biol Anim, 54:770-778, 2018
7. Kito F, Oyama R, Takahashi M, Shiozawa K, Sakumoto M, Yoshida A, Setsu N, Kobayashi E, Kawai A, Kondo T. Establishment and characterization of a patient-derived cancer model of undifferentiated pleomorphic sarcoma. Tiss Cult Res Commun, 37:133-145, 2018