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Home > Organization > Divisions and Independent Research Units > Division of Carcinogenesis and Prevention > Viral Carcinogenesis and Prevention Group > Research Papers

Research Papers

2017

  1. Kayama K, Watanabe S, Takafuji T, Tsuji T, Hironaka K, Matsumoto M, Nakayama K I, Enari M, Kohno T, Shiraishi K, Kiyono T, Yoshida K, Sugimoto N, and Fujita M: GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO reports.18:123-137, 2017. https://www.ncbi.nlm.nih.gov/pubmed/27856536
  2. Katayama M, Hirayama T, Kiyono T, Onuma M, Tani T, Takeda S, Nishimori K, and Fukuda T: Immortalized prairie vole-derived fibroblasts (VMF-K4DTs) can be transformed into pluripotent stem cells and provide a useful tool with which to determine optimal reprogramming conditions. J Reprod Dev. 2017. https://www.ncbi.nlm.nih.gov/pubmed/28331164
  3. Hashimoto H, Suda Y, Miyashita T, Ochiai A, Tsuboi M, Masutomi K, Kiyono T, and Ishii G: A novel method to generate single-cell-derived cancer-associated fibroblast clones. J Cancer Res Clin Oncol. 2017. https://www.ncbi.nlm.nih.gov/pubmed/28364361
  4. Yoshimatsu Y, Nakahara T, Tanaka K, Inagawa Y, Narisawa-Saito M, Yugawa T, Ohno S I, Fujita M, Nakagama H, and Kiyono T: Roles of the PDZ-binding motif of HPV 16 E6 protein in oncogenic transformation of human cervical keratinocytes. Cancer Sci. 2017. https://www.ncbi.nlm.nih.gov/pubmed/28440909
  5. Mori S, Takeuchi T, Ishii Y, Yugawa T, Kiyono T, Nishina H, and Kukimoto I: Human Papillomavirus 16 E6 Upregulates APOBEC3B via the TEAD Transcription Factor. J Virol.91, 2017. https://www.ncbi.nlm.nih.gov/pubmed/28077648
  6. Shinohara M, Sumino Y, Sato F, Kiyono T, Hashimoto N, and Mimata H: Tumor necrosis factor-alpha inhibits differentiation of myogenic cells in human urethral rhabdosphincter. Int J Urol. 2017.https://www.ncbi.nlm.nih.gov/pubmed/28332237
  7. Yokoi A, Yoshioka Y, Yamamoto Y, Ishikawa M, Ikeda S I, Kato T, Kiyono T, Takeshita F, Kajiyama H, Kikkawa F, and Ochiya T: Malignant extracellular vesicles carrying MMP1 mRNA facilitate peritoneal dissemination in ovarian cancer. Nature communications.8:14470, 2017. https://www.ncbi.nlm.nih.gov/pubmed/28262727

2016

  1. Yoshida M, Taguchi A, Kawana K, Adachi K, Kawata A, Ogishima J, Nakamura H, Fujimoto A, Sato M, Inoue T, Nishida H, Furuya H, Tomio K, Arimoto T, Koga K, Wada-Hiraike O, Oda K, Nagamatsu T, Kiyono T, Osuga Y, and Fujii T: Modification of the Tumor Microenvironment in KRAS or c-MYC-Induced Ovarian Cancer-Associated Peritonitis. PloS one.11:e0160330, 2016. https://www.ncbi.nlm.nih.gov/pubmed/27483433
  2. Yachida S, Wood L D, Suzuki M, Takai E, Totoki Y, Kato M, Luchini C, Arai Y, Nakamura H, Hama N, Elzawahry A, Hosoda F, Shirota T, Morimoto N, Hori K, Funazaki J, Tanaka H, Morizane C, Okusaka T, Nara S, Shimada K, Hiraoka N, Taniguchi H, Higuchi R, Oshima M, Okano K, Hirono S, Mizuma M, Arihiro K, Yamamoto M, Unno M, Yamaue H, Weiss M J, Wolfgang C L, Furukawa T, Nakagama H, Vogelstein B, Kiyono T, Hruban R H, and Shibata T: Genomic Sequencing Identifies ELF3 as a Driver of Ampullary Carcinoma. Cancer cell.29:229-240, 2016. https://www.ncbi.nlm.nih.gov/pubmed/26806338
  3. Tone H, Yoshioka S, Akiyama H, Nishimura A, Ichimura M, Nakatani M, Kiyono T, Toyoda M, Watanabe M, and Umezawa A: Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform. BioMed research international.2016:7098987, 2016. https://www.ncbi.nlm.nih.gov/pubmed/27648449
  4. Pantic B, Borgia D, Giunco S, Malena A, Kiyono T, Salvatori S, De Rossi A, Giardina E, Sangiuolo F, Pegoraro E, Vergani L, and Botta A: Reliable and versatile immortal muscle cell models from healthy and myotonic dystrophy type 1 primary human myoblasts. Exp Cell Res.342:39-51, 2016. https://www.ncbi.nlm.nih.gov/pubmed/26905645
  5. Mizushima T, Asai-Sato M, Akimoto K, Nagashima Y, Taguri M, Sasaki K, Nakaya M A, Asano R, Tokinaga A, Kiyono T, Hirahara F, Ohno S, and Miyagi E: Aberrant Expression of the Cell Polarity Regulator aPKClambda/iota is Associated With Disease Progression in Cervical Intraepithelial Neoplasia (CIN): A Possible Marker for Predicting CIN Prognosis. International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists.35:106-117, 2016. https://www.ncbi.nlm.nih.gov/pubmed/26535980
  6. Kondo T, Ozawa S, Ikoma T, Yang X Y, Kanamori K, Suzuki K, Iwabuchi H, Maehata Y, Miyamoto C, Taguchi T, Kiyono T, Kubota E, and Hata R I: Expression of the chemokine CXCL14 and cetuximab-dependent tumour suppression in head and neck squamous cell carcinoma. Oncogenesis.5:e240, 2016. https://www.ncbi.nlm.nih.gov/pubmed/27399917
  7. Kinebuchi M, Matsuura A, Kiyono T, Nomura Y, and Kimura S: Diagnostic copper imaging of Menkes disease by synchrotron radiation-generated X-ray fluorescence analysis. Scientific reports.6:33247, 2016. https://www.ncbi.nlm.nih.gov/pubmed/27629586
  8. Katayama M, Kiyono T, Horie K, Hirayama T, Eitsuka T, Kuroda K, Donai K, Hidema S, Nishimori K, and Fukuda T: Establishment of an immortalized cell line derived from the prairie vole via lentivirus-mediated transduction of mutant cyclin-dependent kinase 4, cyclin D, and telomerase reverse transcriptase. Experimental animals / Japanese Association for Laboratory Animal Science.65:87-96, 2016. https://www.ncbi.nlm.nih.gov/pubmed/26496927
  9. Katayama M, Hirayama T, Horie K, Kiyono T, Donai K, Takeda S, Nishimori K, and Fukuda T: Induced Pluripotent Stem Cells With Six Reprogramming Factors From Prairie Vole, Which Is an Animal Model for Social Behaviors. Cell Transplant.25:783-796, 2016. https://www.ncbi.nlm.nih.gov/pubmed/26777120
  10. Kanda T, Furuse Y, Oshitani H, and Kiyono T: Highly Efficient CRISPR/Cas9-Mediated Cloning and Functional Characterization of Gastric Cancer-Derived Epstein-Barr Virus Strains. J Virol.90:4383-4393, 2016. https://www.ncbi.nlm.nih.gov/pubmed/26889033
  11. Kakiuchi T, Takahara T, Kasugai Y, Arita K, Yoshida N, Karube K, Suguro M, Matsuo K, Nakanishi H, Kiyono T, Nakamura S, Osada H, Sekido Y, Seto M, and Tsuzuki S: Modeling mesothelioma utilizing human mesothelial cells reveals involvement of phospholipase-C beta 4 in YAP-active mesothelioma cell proliferation. Carcinogenesis. 2016. https://www.ncbi.nlm.nih.gov/pubmed/27559111
  12. Iwata Y, Suzuki N, Ohtake H, Kamauchi S, Hashimoto N, Kiyono T, and Wakabayashi S: Cancer cachexia causes skeletal muscle damage via transient receptor potential vanilloid 2-independent mechanisms, unlike muscular dystrophy. Journal of cachexia, sarcopenia and muscle.7:366-376, 2016. https://www.ncbi.nlm.nih.gov/pubmed/27239414
  13. Fukuda T, Iino Y, Onuma M, Gen B, Inoue-Murayama M, and Kiyono T: Expression of human cell cycle regulators in the primary cell line of the African savannah elephant (loxodonta africana) increases proliferation until senescence, but does not induce immortalization. In vitro cellular & developmental biology. Animal.52:20-26, 2016. https://www.ncbi.nlm.nih.gov/pubmed/26487427
  14. Fukuda T, Iino Y, Eitsuka T, Onuma M, Katayama M, Murata K, Inoue-Murayama M, Hara K, Isogai E, and Kiyono T: Cellular conservation of endangered midget buffalo (Lowland Anoa, Bubalus quarlesi) by establishment of primary cultured cell, and its immortalization with expression of cell cycle regulators. Cytotechnology.68:1937-1947, 2016. https://www.ncbi.nlm.nih.gov/pubmed/27449922
  15. Chopjitt P, Pientong C, Sunthamala N, Kongyingyoes B, Haonon O, Boonmars T, Kikawa S, Nakahara T, Kiyono T, and Ekalaksananan T: E6D25E, HPV16 Asian variant shows specific proteomic pattern correlating in cells transformation and suppressive innate immune response. Biochem Biophys Res Commun.478:417-423, 2016. https://www.ncbi.nlm.nih.gov/pubmed/27392712
  16. Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, Yamano S, Wanibuchi H, He D, Goshima N, Kiyono T, Hirotsune S, and Inagaki M: Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. J Cell Biol.212:409-423, 2016. https://www.ncbi.nlm.nih.gov/pubmed/26880200
  17. Nakahara T and Kiyono T: Interplay between NFκB/Interferon (IFN) signaling and the genome replication of human papillomavirus. Future Virology 11(2):141-155, 2016 (review). http://www.futuremedicine.com/doi/full/10.2217/fvl.16.2
  18. Nakahara T and Kiyono T: Molecular mechanisms for HPV persistence. Igaku-no-Ayumi 258(2):133-138 Review.https://www.ishiyaku.co.jp/magazines/ayumi/AyumiArticleDetail.aspx?BC=925802&AC=16352

2015

  1. Takeshima H, Niwa T, Takahashi T, Wakabayashi M, Yamashita S, Ando T, Inagawa Y, Taniguchi H, Katai H, Sugiyama T, Kiyono T, and Ushijima T: Frequent involvement of chromatin remodeler alterations in gastric field cancerization. Cancer letters.357:328-338, 2015. https://www.ncbi.nlm.nih.gov/pubmed/25462860
  2. Sugimoto N, Maehara K, Yoshida K, Yasukouchi S, Osano S, Watanabe S, Aizawa M, Yugawa T, Kiyono T, Kurumizaka H, Ohkawa Y, and Fujita M: Cdt1-binding protein GRWD1 is a novel histone-binding protein that facilitates MCM loading through its influence on chromatin architecture. Nucleic acids research.43:5898-5911, 2015. https://www.ncbi.nlm.nih.gov/pubmed/25990725
  3. Ohira M, Iwasaki Y, Tanaka C, Kuroki M, Matsuo N, Kitamura T, Yukuhiro M, Morimoto H, Pang N, Liu B, Kiyono T, Amemiya M, Tanaka K, Yoshida K, Sugimoto N, Ohshima T, and Fujita M: A novel anti-microtubule agent with carbazole and benzohydrazide structures suppresses tumor cell growth in vivo. Biochim Biophys Acta.1850:1676-1684, 2015. https://www.ncbi.nlm.nih.gov/pubmed/25960391
  4. Nakamura T, Iwase A, Bayasula B, Nagatomo Y, Kondo M, Nakahara T, Takikawa S, Goto M, Kotani T, Kiyono T, and Kikkawa F: CYP51A1 induced by growth differentiation factor 9 and follicle-stimulating hormone in granulosa cells is a possible predictor for unfertilization. Reprod Sci.22:377-384, 2015. https://www.ncbi.nlm.nih.gov/pubmed/24711211
  5. Nakahara T, Tanaka K, Ohno S, Egawa N, Yugawa T, and Kiyono T: Activation of NF-kappaB by human papillomavirus 16 E1 limits E1-dependent viral replication through degradation of E1. J Virol.89:5040-5059, 2015. https://www.ncbi.nlm.nih.gov/pubmed/25717108
  6. Kuroda K, Kiyono T, Isogai E, Masuda M, Narita M, Okuno K, Koyanagi Y, and Fukuda T: Immortalization of Fetal Bovine Colon Epithelial Cells by Expression of Human Cyclin D1, Mutant Cyclin Dependent Kinase 4, and Telomerase Reverse Transcriptase: An In Vitro Model for Bacterial Infection. PloS one.10:e0143473, 2015. https://www.ncbi.nlm.nih.gov/pubmed/26624883
  7. Kuroda K, Kiyono T, Eitsuka T, Isogai H, Takahashi K, Donai K, Isogai E, and Fukuda T: Establishment of cell lines derived from the genus Macaca through controlled expression of cell cycle regulators. Journal of cellular biochemistry.116:205-211, 2015. https://www.ncbi.nlm.nih.gov/pubmed/25187009
  8. Ichioka M, Mita S, Shimizu Y, Imada K, Kiyono T, Bono Y, and Kyo S: Dienogest, a synthetic progestin, down-regulates expression of CYP19A1 and inflammatory and neuroangiogenesis factors through progesterone receptor isoforms A and B in endometriotic cells. The Journal of steroid biochemistry and molecular biology.147:103-110, 2015.https://www.ncbi.nlm.nih.gov/pubmed/25533385
  9. Hata R, Izukuri K, Kato Y, Sasaki S, Mukaida N, Maehata Y, Miyamoto C, Akasaka T, Yang X, Nagashima Y, Takeda K, Kiyono T, and Taniguchi M: Suppressed rate of carcinogenesis and decreases in tumour volume and lung metastasis in CXCL14/BRAK transgenic mice. Scientific reports.5:9083, 2015. https://www.ncbi.nlm.nih.gov/pubmed/25765541

2014

  1. Otsubo C, Otomo R, Miyazaki M, Matsushima-Hibiya Y, Kohno T, Iwakawa R, Takeshita F, Okayama H, Ichikawa H, Saya H, Kiyono T, Ochiya T, Tashiro F, Nakagama H, Yokota J, and Enari M: TSPAN2 is involved in cell invasion and motility during lung cancer progression. Cell reports.7:527-538, 2014. https://www.ncbi.nlm.nih.gov/pubmed/24726368
  2. Kasahara K, Kawakami Y, Kiyono T, Yonemura S, Kawamura Y, Era S, Matsuzaki F, Goshima N, and Inagaki M: Ubiquitin-proteasome system controls ciliogenesis at the initial step of axoneme extension. Nature communications.5:5081, 2014. https://www.ncbi.nlm.nih.gov/pubmed/25270598
  3. Iwahori S, Kohmon D, Kobayashi J, Tani Y, Yugawa T, Komatsu K, Kiyono T, Sugimoto N, and Fujita M: ATM regulates Cdt1 stability during the unperturbed S phase to prevent re-replication. Cell Cycle.13:471-481, 2014. https://www.ncbi.nlm.nih.gov/pubmed/24280901
  4. Fuchigami T, Kibe T, Koyama H, Kishida S, Iijima M, Nishizawa Y, Hijioka H, Fujii T, Ueda M, Nakamura N, Kiyono T, and Kishida M: Regulation of IL-6 and IL-8 production by reciprocal cell-to-cell interactions between tumor cells and stromal fibroblasts through IL-1alpha in ameloblastoma. Biochem Biophys Res Commun.451:491-496, 2014. https://www.ncbi.nlm.nih.gov/pubmed/25124663
  5. Donai K, Kiyono T, Eitsuka T, Guo Y, Kuroda K, Sone H, Isogai E, and Fukuda T: Bovine and porcine fibroblasts can be immortalized with intact karyotype by the expression of mutant cyclin dependent kinase 4, cyclin D, and telomerase. Journal of biotechnology.176:50-57, 2014. https://www.ncbi.nlm.nih.gov/pubmed/24589663
  6. Chihara D, Kagami Y, Kato H, Yoshida N, Kiyono T, Okada Y, Kinoshita T, and Seto M: IL2/IL-4, OX40L and FDC-like cell line support the in vitro tumor cell growth of adult T-cell leukemia/lymphoma. Leukemia research.38:608-612, 2014. https://www.ncbi.nlm.nih.gov/pubmed/24679586
  7. Bono Y, Kyo S, Kiyono T, Mizumoto Y, Nakamura M, Maida Y, Takakura M, and Fujiwara H: Concurrent estrogen action was essential for maximal progestin effect in oral contraceptives. Fertility and sterility.101:1337-1343, 2014. https://www.ncbi.nlm.nih.gov/pubmed/24656887
  8. Inagawa Y, Yamada K, Yugawa T, Ohno S, Hiraoka N, Esaki M, Shibata T, Aoki K, Saya H, and Kiyono T: A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes. Carcinogenesis.35:1840-1846, 2014. https://www.ncbi.nlm.nih.gov/pubmed/24858378
  9. Uno M, Saitoh Y, Mochida K, Tsuruyama E, Kiyono T, Imoto I, Inazawa J, Yuasa Y, Kubota T, and Yamaoka S: NF-kappaB inducing kinase, a central signaling component of the non-canonical pathway of NF-kappaB, contributes to ovarian cancer progression. PloS one.9:e88347, 2014. https://www.ncbi.nlm.nih.gov/pubmed/24533079
  10. Uekita T, Fujii S, Miyazawa Y, Iwakawa R, Narisawa-Saito M, Nakashima K, Tsuta K, Tsuda H, Kiyono T, Yokota J, and Sakai R: Oncogenic Ras/ERK signaling activates CDCP1 to promote tumor invasion and metastasis. Molecular cancer research : MCR.12:1449-1459, 2014. https://www.ncbi.nlm.nih.gov/pubmed/24939643
  11. Shiomi K, Nagata Y, Kiyono T, Harada A, and Hashimoto N: Differential impact of the bisphosphonate alendronate on undifferentiated and terminally differentiated human myogenic cells. The Journal of pharmacy and pharmacology.66:418-427, 2014. https://www.ncbi.nlm.nih.gov/pubmed/24533824
  12. Nakahara T and Kiyono T: Regulation of human papillomavirus (HPV) genome replication in the viral life cycle and its association with the viral persistence and cancer development. Uirusu. 64(1):57-66, 2014. Review. Japanese. https://www.ncbi.nlm.nih.gov/pubmed/25765981

2013

  1. Teng Z, Yoshida T, Okabe M, Toda A, Higuchi O, Nogami M, Yoneda N, Zhou K, Kyo S, Kiyono T, and Nikaido T: Establishment of immortalized human amniotic mesenchymal stem cells. Cell Transplant.22:267-278, 2013. https://www.ncbi.nlm.nih.gov/pubmed/23006979
  2. Nakamura M, Takakura M, Fujii R, Maida Y, Bono Y, Mizumoto Y, Zhang X, Kiyono T, and Kyo S: The PRB-dependent FOXO1/IGFBP-1 axis is essential for progestin to inhibit endometrial epithelial growth. Cancer letters.336:68-75, 2013. https://www.ncbi.nlm.nih.gov/pubmed/23603247
  3. Mitsuishi T, Ohsawa I, Kato T, Egawa N, and Kiyono T: Molecular cloning and characterisation of a novel type of human papillomavirus 160 isolated from a flat wart of an immunocompetent patient. PloS one.8:e79592, 2013. https://www.ncbi.nlm.nih.gov/pubmed/24282509
  4. Kibe T, Fuchigami T, Kishida M, Iijima M, Ishihata K, Hijioka H, Miyawaki A, Semba I, Nakamura N, Kiyono T, and Kishida S: A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis. Oral surgery, oral medicine, oral pathology and oral radiology.115:780-788, 2013. https://www.ncbi.nlm.nih.gov/pubmed/23706918
  5. Kasahara K, Goto H, Izawa I, Kiyono T, Watanabe N, Elowe S, Nigg E A, and Inagaki M: PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3gamma and is required for metaphase-anaphase transition. Nature communications.4:1882, 2013. https://www.ncbi.nlm.nih.gov/pubmed/23695676
  6. Zhou K, Koike C, Yoshida T, Okabe M, Fathy M, Kyo S, Kiyono T, Saito S, and Nikaido T: Establishment and characterization of immortalized human amniotic epithelial cells. Cellular reprogramming.15:55-67, 2013. https://www.ncbi.nlm.nih.gov/pubmed/23298399
  7. Yugawa T, Nishino K, Ohno S, Nakahara T, Fujita M, Goshima N, Umezawa A, and Kiyono T: Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol Cell Biol.33:4434-4447, 2013. https://www.ncbi.nlm.nih.gov/pubmed/24019071
  8. Unno J, Takagi M, Piao J, Sugimoto M, Honda F, Maeda D, Masutani M, Kiyono T, Watanabe F, Morio T, Teraoka H, and Mizutani S: Artemis-dependent DNA double-strand break formation at stalled replication forks. Cancer Sci.104:703-710, 2013. https://www.ncbi.nlm.nih.gov/pubmed/23465063
  9. Tokuda N, Numata S, Li X, Nomura T, Takizawa M, Kondo Y, Yamashita Y, Hashimoto N, Kiyono T, Urano T, Furukawa K, and Furukawa K: beta4GalT6 is involved in the synthesis of lactosylceramide with less intensity than beta4GalT5. Glycobiology.23:1175-1183, 2013. https://www.ncbi.nlm.nih.gov/pubmed/23882130
  10. Ishii Y, Nakahara T, Kataoka M, Kusumoto-Matsuo R, Mori S, Takeuchi T, Kukimoto I: Identification of TRAPPC8 as a host factor required for human papillomavirus entry. PLoS One 8(11):e80297, 2013. https://www.ncbi.nlm.nih.gov/pubmed/24244674

Before 2012

  1. Yokoi T, Seko Y, Yokoi T, Makino H, Hatou S, Yamada M, Kiyono T, Umezawa A, Nishina H, and Azuma N: Establishment of functioning human corneal endothelial cell line with high growth potential. PloS one.7:e29677, 2012. https://www.ncbi.nlm.nih.gov/pubmed/22276123
  2. Nishimoto N, Watanabe M, Watanabe S, Sugimoto N, Yugawa T, Ikura T, Koiwai O, Kiyono T, and Fujita M: Heterocomplex formation by Arp4 and beta-actin is involved in the integrity of the Brg1 chromatin remodeling complex. J Cell Sci.125:3870-3882, 2012. https://www.ncbi.nlm.nih.gov/pubmed/22573825
  3. Narisawa-Saito M, Inagawa Y, Yoshimatsu Y, Haga K, Tanaka K, Egawa N, Ohno S, Ichikawa H, Yugawa T, Fujita M, and Kiyono T: A critical role of MYC for transformation of human cells by HPV16 E6E7 and oncogenic HRAS. Carcinogenesis.33:910-917, 2012. https://www.ncbi.nlm.nih.gov/pubmed/22345164
  4. Li P, Goto H, Kasahara K, Matsuyama M, Wang Z, Yatabe Y, Kiyono T, and Inagaki M: P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation. Mol Biol Cell.23:1582-1592, 2012. https://www.ncbi.nlm.nih.gov/pubmed/22357623
  5. Inoko A, Matsuyama M, Goto H, Ohmuro-Matsuyama Y, Hayashi Y, Enomoto M, Ibi M, Urano T, Yonemura S, Kiyono T, Izawa I, and Inagaki M: Trichoplein and Aurora A block aberrant primary cilia assembly in proliferating cells. J Cell Biol.197:391-405, 2012. https://www.ncbi.nlm.nih.gov/pubmed/22529102
  6. Egawa N, Kawai K, Egawa K, Honda Y, Kanekura T, and Kiyono T: Molecular cloning and characterization of a novel human papillomavirus, HPV 126, isolated from a flat wart-like lesion with intracytoplasmic inclusion bodies and a peculiar distribution of Ki-67 and p53. Virology.422:99-104, 2012. https://www.ncbi.nlm.nih.gov/pubmed/22056388
  7. Deardorff M A, Bando M, Nakato R, Watrin E, Itoh T, Minamino M, Saitoh K, Komata M, Katou Y, Clark D, Cole K E, De Baere E, Decroos C, Di Donato N, Ernst S, Francey L J, Gyftodimou Y, Hirashima K, Hullings M, Ishikawa Y, Jaulin C, Kaur M, Kiyono T, Lombardi P M, Magnaghi-Jaulin L, Mortier G R, Nozaki N, Petersen M B, Seimiya H, Siu V M, Suzuki Y, Takagaki K, Wilde J J, Willems P J, Prigent C, Gillessen-Kaesbach G, Christianson D W, Kaiser F J, Jackson L G, Hirota T, Krantz I D, and Shirahige K: HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle. Nature.489:313-317, 2012. https://www.ncbi.nlm.nih.gov/pubmed/22885700
  8. Bayasula, Iwase A, Kiyono T, Takikawa S, Goto M, Nakamura T, Nagatomo Y, Nakahara T, Kotani T, Kobayashi H, Kondo M, Manabe S, and Kikkawa F: Establishment of a human nonluteinized granulosa cell line that transitions from the gonadotropin-independent to the gonadotropin-dependent status. Endocrinology.153:2851-2860, 2012. https://www.ncbi.nlm.nih.gov/pubmed/22467494
  9. Egawa N, Nakahara T, Ohno S, Narisawa-Saito M, Yugawa T, Fujita M, Yamato K, Natori Y, and Kiyono T: The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome. J Virol.86:3276-3283, 2012. https://www.ncbi.nlm.nih.gov/pubmed/22238312
  10. Bono Y, Kyo S, Takakura M, Maida Y, Mizumoto Y, Nakamura M, Nomura K, Kiyono T, and Inoue M: Creation of immortalised epithelial cells from ovarian endometrioma. Br J Cancer.106:1205-1213, 2012. https://www.ncbi.nlm.nih.gov/pubmed/22353808
  11. Zushi Y, Narisawa-Saito M, Noguchi K, Yoshimatsu Y, Yugawa T, Egawa N, Fujita M, Urade M, and Kiyono T: An in vitro multistep carcinogenesis model for both HPV-positive and -negative human oral squamous cell carcinomas. Am J Cancer Res.1:869-881, 2011. https://www.ncbi.nlm.nih.gov/pubmed/22016834
  12. Yamato K, Egawa N, Endo S, Ui-Tei K, Yamada T, Saigo K, Hyodo I, Kiyono T, and Nakagawa I: Enhanced specificity of HPV16 E6E7 siRNA by RNA-DNA chimera modification. Cancer Gene Ther.18:587-597, 2011. https://www.ncbi.nlm.nih.gov/pubmed/21660064
  13. Sugimoto N, Yugawa T, Iizuka M, Kiyono T, and Fujita M: Chromatin remodeler sucrose nonfermenting 2 homolog (SNF2H) is recruited onto DNA replication origins through interaction with Cdc10 protein-dependent transcript 1 (Cdt1) and promotes pre-replication complex formation. J Biol Chem.286:39200-39210, 2011. https://www.ncbi.nlm.nih.gov/pubmed/21937426
  14. Shiomi K, Kiyono T, Okamura K, Uezumi M, Goto Y, Yasumoto S, Shimizu S, and Hashimoto N: CDK4 and cyclin D1 allow human myogenic cells to recapture growth property without compromising differentiation potential. Gene Ther.18:857-866, 2011. https://www.ncbi.nlm.nih.gov/pubmed/21490680
  15. Shibata T, Kokubu A, Saito S, Narisawa-Saito M, Sasaki H, Aoyagi K, Yoshimatsu Y, Tachimori Y, Kushima R, Kiyono T, and Yamamoto M: NRF2 mutation confers malignant potential and resistance to chemoradiation therapy in advanced esophageal squamous cancer. Neoplasia.13:864-873, 2011. https://www.ncbi.nlm.nih.gov/pubmed/21969819
  16. Shaker M, Yokoyama Y, Mori S, Tsujimoto M, Kawaguchi N, Kiyono T, Nakano T, and Matsuura N: Aberrant expression of disintegrin-metalloprotease proteins in the formation and progression of uterine cervical cancer. Pathobiology.78:149-161, 2011. https://www.ncbi.nlm.nih.gov/pubmed/21613802
  17. Matsuyama M, Goto H, Kasahara K, Kawakami Y, Nakanishi M, Kiyono T, Goshima N, and Inagaki M: Nuclear Chk1 prevents premature mitotic entry. J Cell Sci.124:2113-2119, 2011. https://www.ncbi.nlm.nih.gov/pubmed/21628425
  18. Kyo S, Sakaguchi J, Kiyono T, Shimizu Y, Maida Y, Mizumoto Y, Mori N, Nakamura M, Takakura M, Miyake K, Sakamoto M, and Inoue M: Forkhead transcription factor FOXO1 is a direct target of progestin to inhibit endometrial epithelial cell growth. Clin Cancer Res.17:525-537, 2011. https://www.ncbi.nlm.nih.gov/pubmed/21131554
  19. Koga K, Aoki M, Sameshima T, Hamasaki M, Egawa N, Seiki M, Toole B P, Suzumiya J, and Nabeshima K: Synthetic emmprin peptides inhibit tumor cell-fibroblast interaction-stimulated upregulation of MMP-2 and tumor cell invasion. Int J Oncol.39:657-664, 2011. https://www.ncbi.nlm.nih.gov/pubmed/21637915
  20. Ibi M, Zou P, Inoko A, Shiromizu T, Matsuyama M, Hayashi Y, Enomoto M, Mori D, Hirotsune S, Kiyono T, Tsukita S, Goto H, and Inagaki M: Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein. J Cell Sci.124:857-864, 2011. https://www.ncbi.nlm.nih.gov/pubmed/21325031
  21. Fujiwara S, Nawa A, Luo C, Kamakura M, Goshima F, Kondo C, Kiyono T, Kikkawa F, and Nishiyama Y: Carrier cell-based delivery of replication-competent HSV-1 mutants enhances antitumor effect for ovarian cancer. Cancer Gene Ther.18:77-86, 2011. https://www.ncbi.nlm.nih.gov/pubmed/20885447
  22. Mizumoto Y, Kyo S, Kiyono T, Takakura M, Nakamura M, Maida Y, Mori N, Bono Y, Sakurai H, and Inoue M: Activation of NF-kappaB is a novel target of KRAS-induced endometrial carcinogenesis. Clin Cancer Res.17:1341-1350, 2011. https://www.ncbi.nlm.nih.gov/pubmed/21411444
  23. Kasahara K, Goto H, Enomoto M, Tomono Y, Kiyono T, and Inagaki M: 14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage. EMBO J.29:2802-2812, 2010. https://www.ncbi.nlm.nih.gov/pubmed/20639859
  24. Kiyono T: [Papillomavirus infections and cervical tumorigenesis]. Gan To Kagaku Ryoho.37:227-231, 2010. https://www.ncbi.nlm.nih.gov/pubmed/20235389
  25. Mori N, Kyo S, Nakamura M, Hashimoto M, Maida Y, Mizumoto Y, Takakura M, Ohno S, Kiyono T, and Inoue M: Expression of HER-2 affects patient survival and paclitaxel sensitivity in endometrial cancer. Br J Cancer.103:889-898, 2010. https://www.ncbi.nlm.nih.gov/pubmed/20664599
  26. Roy B C, Kohno T, Iwakawa R, Moriguchi T, Kiyono T, Morishita K, Sanchez-Cespedes M, Akiyama T, and Yokota J: Involvement of LKB1 in epithelial-mesenchymal transition (EMT) of human lung cancer cells. Lung Cancer.70:136-145, 2010. https://www.ncbi.nlm.nih.gov/pubmed/20207041
  27. Yoshida K, Sugimoto N, Iwahori S, Yugawa T, Narisawa-Saito M, Kiyono T, and Fujita M: CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells. J Cell Sci.123:225-235, 2010. https://www.ncbi.nlm.nih.gov/pubmed/20048340
  28. Yugawa T, Narisawa-Saito M, Yoshimatsu Y, Haga K, Ohno S, Egawa N, Fujita M, and Kiyono T: DeltaNp63alpha repression of the Notch1 gene supports the proliferative capacity of normal human keratinocytes and cervical cancer cells. Cancer Res.70:4034-4044, 2010. https://www.ncbi.nlm.nih.gov/pubmed/20442293
  29. Aoyagi T, Takahashi M, Higuchi M, Oie M, Tanaka Y, Kiyono T, Aoyagi Y, and Fujii M: The PDZ domain binding motif (PBM) of human T-cell leukemia virus type 1 Tax can be substituted by heterologous PBMs from viral oncoproteins during T-cell transformation. Virus Genes.40:193-199, 2010. https://www.ncbi.nlm.nih.gov/pubmed/20069350
  30. Egawa N, and Kiyono T: [HPV DNA testing and application for cervical cancer]. Nihon Rinsho.68 Suppl 8:535-540, 2010. https://www.ncbi.nlm.nih.gov/pubmed/20976929
  31. Ito S, Ozawa S, Ikoma T, Yajima N, Kiyono T, and Hata R: Expression of a chemokine BRAK/CXCL14 in oral floor carcinoma cells reduces the settlement rate of the cells and suppresses their proliferation in vivo. Biomed Res.31:199-206, 2010. https://www.ncbi.nlm.nih.gov/pubmed/20622470
  32. Iwakawa R, Kohno T, Enari M, Kiyono T, and Yokota J: Prevalence of human papillomavirus 16/18/33 infection and p53 mutation in lung adenocarcinoma. Cancer Sci.101:1891-1896, 2010. https://www.ncbi.nlm.nih.gov/pubmed/20557307
  33. Sasaki R, Narisawa-Saito M, Yugawa T, Fujita M, Tashiro H, Katabuchi H, and Kiyono T: Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes. Carcinogenesis.30:423-431, 2009. https://www.ncbi.nlm.nih.gov/pubmed/19126650
  34. Omi H, Okamoto A, Nikaido T, Urashima M, Kawaguchi R, Umehara N, Sugiura K, Saito M, Kiyono T, and Tanaka T: Establishment of an immortalized human extravillous trophoblast cell line by retroviral infection of E6/E7/hTERT and its transcriptional profile during hypoxia and reoxygenation. Int J Mol Med.23:229-236, 2009. https://www.ncbi.nlm.nih.gov/pubmed/19148547
  35. Narisawa-Saito M, and Kiyono T: [Mechanisms of high-risk human papillomavirus-induced cervical carcinogenesis]. Nihon Rinsho.67:53-61, 2009. https://www.ncbi.nlm.nih.gov/pubmed/19177752
  36. Kawai K, Egawa N, Kiyono T, and Kanekura T: Epidermodysplasia-verruciformis-like eruption associated with gamma-papillomavirus infection in a patient with adult T-cell leukemia. Dermatology.219:274-278, 2009. https://www.ncbi.nlm.nih.gov/pubmed/19696474
  37. Ibrahim H R, and Kiyono T: Novel anticancer activity of the autocleaved ovotransferrin against human colon and breast cancer cells. J Agric Food Chem.57:11383-11390, 2009. https://www.ncbi.nlm.nih.gov/pubmed/19886663
  38. Enomoto M, Goto H, Tomono Y, Kasahara K, Tsujimura K, Kiyono T, and Inagaki M: Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during G2/M transition. J Biol Chem.284:34223-34230, 2009. https://www.ncbi.nlm.nih.gov/pubmed/19837665
  39. Sugimoto N, Yoshida K, Tatsumi Y, Yugawa T, Narisawa-Saito M, Waga S, Kiyono T, and Fujita M: Redundant and differential regulation of multiple licensing factors ensures prevention of re-replication in normal human cells. J Cell Sci.122:1184-1191, 2009. https://www.ncbi.nlm.nih.gov/pubmed/19339550
  40. Shimizu Y, Takeuchi T, Mita S, Mizuguchi K, Kiyono T, Inoue M, and Kyo S: Dienogest, a synthetic progestin, inhibits the proliferation of immortalized human endometrial epithelial cells with suppression of cyclin D1 gene expression. Mol Hum Reprod.15:693-701, 2009. https://www.ncbi.nlm.nih.gov/pubmed/19502247
  41. Yugawa T, and Kiyono T: Molecular mechanisms of cervical carcinogenesis by high-risk human papillomaviruses: novel functions of E6 and E7 oncoproteins. Rev Med Virol.19:97-113, 2009. https://www.ncbi.nlm.nih.gov/pubmed/19156753
  42. Yuan H, Ito S, Senga T, Hyodo T, Kiyono T, Kikkawa F, and Hamaguchi M: Human papillomavirus type 16 oncoprotein E7 suppresses cadherin-mediated cell adhesion via ERK and AP-1 signaling. Int J Oncol.35:309-314, 2009. https://www.ncbi.nlm.nih.gov/pubmed/19578744
  43. Tsuruga Y, Kiyono T, Matsushita M, Takahashi T, Kasai N, Matsumoto S, and Todo S: Effect of intrasplenic transplantation of immortalized human hepatocytes in the treatment of acetaminophen-induced acute liver failure SCID mice. Transplant Proc.40:617-619, 2008. https://www.ncbi.nlm.nih.g ov/pubmed/18374145
  44. Tatsumi Y, Ezura K, Yoshida K, Yugawa T, Narisawa-Saito M, Kiyono T, Ohta S, Obuse C, and Fujita M: Involvement of human ORC and TRF2 in pre-replication complex assembly at telomeres. Genes Cells.13:1045-1059, 2008. https://www.ncbi.nlm.nih.gov/pubmed/18761675
  45. Sugimoto N, Kitabayashi I, Osano S, Tatsumi Y, Yugawa T, Narisawa-Saito M, Matsukage A, Kiyono T, and Fujita M: Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1. Mol Biol Cell.19:1007-1021, 2008. https://www.ncbi.nlm.nih.gov/pubmed/18162579
  46. Sugimoto M, Inoko A, Shiromizu T, Nakayama M, Zou P, Yonemura S, Hayashi Y, Izawa I, Sasoh M, Uji Y, Kaibuchi K, Kiyono T, and Inagaki M: The keratin-binding protein Albatross regulates polarization of epithelial cells. J Cell Biol.183:19-28, 2008. https://www.ncbi.nlm.nih.gov/pubmed/18838552
  47. Shiga M, Saito M, Hattori M, Torii C, Kosaki K, Kiyono T, and Suda N: Characteristic phenotype of immortalized periodontal cells isolated from a Marfan syndrome type I patient. Cell Tissue Res.331:461-472, 2008. https://www.ncbi.nlm.nih.gov/pubmed/18049824
  48. Scian M J, Carchman E H, Mohanraj L, Stagliano K E, Anderson M A, Deb D, Crane B M, Kiyono T, Windle B, Deb S P, and Deb S: Wild-type p53 and p73 negatively regulate expression of proliferation related genes. Oncogene.27:2583-2593, 2008. https://www.ncbi.nlm.nih.gov/pubmed/17982488
  49. Tsuruga Y, Kiyono T, Matsushita M, Takahashi T, Kasai H, Matsumoto S, and Todo S: Establishment of immortalized human hepatocytes by introduction of HPV16 E6/E7 and hTERT as cell sources for liver cell-based therapy. Cell Transplant.17:1083-1094, 2008. https://www.ncbi.nlm.nih.gov/pubmed/19177844
  50. Narisawa-Saito M, Yoshimatsu Y, Ohno S, Yugawa T, Egawa N, Fujita M, Hirohashi S, and Kiyono T: An in vitro multistep carcinogenesis model for human cervical cancer. Cancer Res.68:5699-5705, 2008. https://www.ncbi.nlm.nih.gov/pubmed/18632622 107. Ikegami Y, Goto H, Kiyono T, Enomoto M, Kasahara K, Tomono Y, Tozawa K, Morita A, Kohri K, and Inagaki M: Chk1 phosphorylation at Ser286 and Ser301 occurs with both stalled DNA replication and damage checkpoint stimulation. Biochem Biophys Res Commun.377:1227-1231, 2008. https://www.ncbi.nlm.nih.gov/pubmed/18983824
  51. Hida N, Nishiyama N, Miyoshi S, Kira S, Segawa K, Uyama T, Mori T, Miyado K, Ikegami Y, Cui C, Kiyono T, Kyo S, Shimizu T, Okano T, Sakamoto M, Ogawa S, and Umezawa A: Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells. Stem Cells.26:1695-1704, 2008. https://www.ncbi.nlm.nih.gov/pubmed/18420831
  52. Hashimoto N, Kiyono T, Wada M R, Umeda R, Goto Y, Nonaka I, Shimizu S, Yasumoto S, and Inagawa-Ogashiwa M: Osteogenic properties of human myogenic progenitor cells. Mech Dev.125:257-269, 2008. https://www.ncbi.nlm.nih.gov/pubmed/18164186
  53. Narisawa-Saito M, and Kiyono T: Basic mechanisms of high-risk human papillomavirus-induced carcinogenesis: roles of E6 and E7 proteins. Cancer Sci.98:1505-1511, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17645777
  54. Narisawa-Saito M, Handa K, Yugawa T, Ohno S, Fujita M, and Kiyono T: HPV16 E6-mediated stabilization of ErbB2 in neoplastic transformation of human cervical keratinocytes. Oncogene.26:2988-2996, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17146442
  55. Morishima S, Akatsuka Y, Nawa A, Kondo E, Kiyono T, Torikai H, Nakanishi T, Ito Y, Tsujimura K, Iwata K, Ito K, Kodera Y, Morishima Y, Kuzushima K, and Takahashi T: Identification of an HLA-A24-restricted cytotoxic T lymphocyte epitope from human papillomavirus type-16 E6: the combined effects of bortezomib and interferon-gamma on the presentation of a cryptic epitope. Int J Cancer.120:594-604, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17096336
  56. Kiyono T: Molecular mechanisms of cellular senescence and immortalization of human cells. Expert Opin Ther Targets.11:1623-1637, 2007. https://www.ncbi.nlm.nih.gov/pubmed/18020982
  57. Ito Y, Kawamorita M, Yamabe T, Kiyono T, and Miyamoto K: Chemically fixed nurse cells for culturing murine or primate embryonic stem cells. J Biosci Bioeng.103:113-121, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17368392
  58. Handa K, Yugawa T, Narisawa-Saito M, Ohno S, Fujita M, and Kiyono T: E6AP-dependent degradation of DLG4/PSD95 by high-risk human papillomavirus type 18 E6 protein. J Virol.81:1379-1389, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17121805
  59. Cui C H, Uyama T, Miyado K, Terai M, Kyo S, Kiyono T, and Umezawa A: Menstrual blood-derived cells confer human dystrophin expression in the murine model of Duchenne muscular dystrophy via cell fusion and myogenic transdifferentiation. Mol Biol Cell.18:1586-1594, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17314403
  60. Yugawa T, Handa K, Narisawa-Saito M, Ohno S, Fujita M, and Kiyono T: Regulation of Notch1 gene expression by p53 in epithelial cells. Mol Cell Biol.27:3732-3742, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17353266
  61. Yokoi T, Saito M, Kiyono T, Iseki S, Kosaka K, Nishida E, Tsubakimoto T, Harada H, Eto K, Noguchi T, and Teranaka T: Establishment of immortalized dental follicle cells for generating periodontal ligament in vivo. Cell Tissue Res.327:301-311, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17013589
  62. Haga K, Ohno S, Yugawa T, Narisawa-Saito M, Fujita M, Sakamoto M, Galloway D A, and Kiyono T: Efficient immortalization of primary human cells by p16INK4a-specific short hairpin RNA or Bmi-1, combined with introduction of hTERT. Cancer Sci.98:147-154, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17233832
  63. Uekita T, Jia L, Narisawa-Saito M, Yokota J, Kiyono T, and Sakai R: CUB domain-containing protein 1 is a novel regulator of anoikis resistance in lung adenocarcinoma. Mol Cell Biol.27:7649-7660, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17785447
  64. Shima Y, Okamoto T, Aoyama T, Yasura K, Ishibe T, Nishijo K, Shibata K R, Kohno Y, Fukiage K, Otsuka S, Uejima D, Nakayama T, Nakamura T, Kiyono T, and Toguchida J: In vitro transformation of mesenchymal stem cells by oncogenic H-rasVal12. Biochem Biophys Res Commun.353:60-66, 2007. https://www.ncbi.nlm.nih.gov/pubmed/17173860
  65. Ito Y, Hasauda H, Kitajima T, and Kiyono T: Ex vivo expansion of human cord blood hematopoietic progenitor cells using glutaraldehyde-fixed human bone marrow stromal cells. J Biosci Bioeng.102:467-469, 2006. https://www.ncbi.nlm.nih.gov/pubmed/17189177
  66. Hashimoto N, Kiyono T, Wada M R, Shimizu S, Yasumoto S, and Inagawa M: Immortalization of human myogenic progenitor cell clone retaining multipotentiality. Biochem Biophys Res Commun.348:1383-1388, 2006. https://www.ncbi.nlm.nih.gov/pubmed/16919240
  67. Goto H, Kiyono T, Tomono Y, Kawajiri A, Urano T, Furukawa K, Nigg E A, and Inagaki M: Complex formation of Plk1 and INCENP required for metaphase-anaphase transition. Nat Cell Biol.8:180-187, 2006. https://www.ncbi.nlm.nih.gov/pubmed/16378098
  68. Yamashita Y, Tsurumi T, Mori N, and Kiyono T: Immortalization of Epstein-Barr virus-negative human B lymphocytes with minimal chromosomal instability. Pathol Int.56:659-667, 2006. https://www.ncbi.nlm.nih.gov/pubmed/17040288
  69. Tatsumi Y, Sugimoto N, Yugawa T, Narisawa-Saito M, Kiyono T, and Fujita M: Deregulation of Cdt1 induces chromosomal damage without rereplication and leads to chromosomal instability. J Cell Sci.119:3128-3140, 2006. https://www.ncbi.nlm.nih.gov/pubmed/16835273
  70. Mizumoto Y, Kyo S, Ohno S, Hashimoto M, Nakamura M, Maida Y, Sakaguchi J, Takakura M, Inoue M, and Kiyono T: Creation of tumorigenic human endometrial epithelial cells with intact chromosomes by introducing defined genetic elements. Oncogene.25:5673-5682, 2006. https://www.ncbi.nlm.nih.gov/pubmed/16636665
  71. Masuda Y, Futamura M, Kamino H, Nakamura Y, Kitamura N, Ohnishi S, Miyamoto Y, Ichikawa H, Ohta T, Ohki M, Kiyono T, Egami H, Baba H, and Arakawa H: The potential role of DFNA5, a hearing impairment gene, in p53-mediated cellular response to DNA damage. J Hum Genet.51:652-664, 2006. https://www.ncbi.nlm.nih.gov/pubmed/16897187
  72. Shirata N, Kudoh A, Daikoku T, Tatsumi Y, Fujita M, Kiyono T, Sugaya Y, Isomura H, Ishizaki K, and Tsurumi T: Activation of ataxia telangiectasia-mutated DNA damage checkpoint signal transduction elicited by herpes simplex virus infection. J Biol Chem.280:30336-30341, 2005. https://www.ncbi.nlm.nih.gov/pubmed/15964848
  73. Saito M, Handa K, Kiyono T, Hattori S, Yokoi T, Tsubakimoto T, Harada H, Noguchi T, Toyoda M, Sato S, and Teranaka T: Immortalization of cementoblast progenitor cells with Bmi-1 and TERT. J Bone Miner Res.20:50-57, 2005. https://www.ncbi.nlm.nih.gov/pubmed/15619669 [
  74. Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, and Umezawa A: Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential. Mol Cell Biol.25:5183-5195, 2005. https://www.ncbi.nlm.nih.gov/pubmed/15923633
  75. Maeda T, Tashiro H, Katabuchi H, Begum M, Ohtake H, Kiyono T, and Okamura H: Establishment of an immortalised human ovarian surface epithelial cell line without chromosomal instability. Br J Cancer.93:116-123, 2005. https://www.ncbi.nlm.nih.gov/pubmed/15956975
  76. Terai M, Uyama T, Sugiki T, Li X K, Umezawa A, and Kiyono T: Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway. Mol Biol Cell.16:1491-1499, 2005. https://www.ncbi.nlm.nih.gov/pubmed/15647378
  77. Kuroda M, Kiyono T, Oikawa K, Yoshida K, and Mukai K: The human papillomavirus E6 and E7 inducible oncogene, hWAPL, exhibits potential as a therapeutic target. Br J Cancer.92:290-293, 2005. https://www.ncbi.nlm.nih.gov/pubmed/15655544
  78. Hara S, Nakashima S, Kiyono T, Sawada M, Yoshimura S, Iwama T, and Sakai N: Ceramide triggers caspase activation during gamma-radiation-induced apoptosis of human glioma cells lacking functional p53. Oncol Rep.12:119-123, 2004. https://www.ncbi.nlm.nih.gov/pubmed/15201971
  79. Hara S, Nakashima S, Kiyono T, Sawada M, Yoshimura S, Iwama T, Banno Y, Shinoda J, and Sakai N: p53-Independent ceramide formation in human glioma cells during gamma-radiation-induced apoptosis. Cell Death Differ.11:853-861, 2004. https://www.ncbi.nlm.nih.gov/pubmed/15088070
  80. Gewin L, Myers H, Kiyono T, and Galloway D A: Identification of a novel telomerase repressor that interacts with the human papillomavirus type-16 E6/E6-AP complex. Genes Dev.18:2269-2282, 2004. https://www.ncbi.nlm.nih.gov/pubmed/15371341
  81. Kudoh A, Daikoku T, Sugaya Y, Isomura H, Fujita M, Kiyono T, Nishiyama Y, and Tsurumi T: Inhibition of S-phase cyclin-dependent kinase activity blocks expression of Epstein-Barr virus immediate-early and early genes, preventing viral lytic replication. J Virol.78:104-115, 2004. https://www.ncbi.nlm.nih.gov/pubmed/14671092
  82. Sugimoto N, Tatsumi Y, Tsurumi T, Matsukage A, Kiyono T, Nishitani H, and Fujita M: Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding. J Biol Chem.279:19691-19697, 2004. https://www.ncbi.nlm.nih.gov/pubmed/14993212
  83. Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, and Umezawa A: Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation? J Gene Med.6:833-845, 2004. https://www.ncbi.nlm.nih.gov/pubmed/15293342
  84. Sawada M, Kiyono T, Nakashima S, Shinoda J, Naganawa T, Hara S, Iwama T, and Sakai N: Molecular mechanisms of TNF-alpha-induced ceramide formation in human glioma cells: P53-mediated oxidant stress-dependent and -independent pathways. Cell Death Differ.11:997-1008, 2004. https://www.ncbi.nlm.nih.gov/pubmed/15131591
  85. Oikawa K, Ohbayashi T, Kiyono T, Nishi H, Isaka K, Umezawa A, Kuroda M, and Mukai K: Expression of a novel human gene, human wings apart-like (hWAPL), is associated with cervical carcinogenesis and tumor progression. Cancer Res.64:3545-3549, 2004. https://www.ncbi.nlm.nih.gov/pubmed/15150110
  86. Kawabe A, Shimada Y, Soma T, Maeda M, Itami A, Kaganoi J, Kiyono T, and Imamura M: Production of prostaglandinE2 via bile acid is enhanced by trypsin and acid in normal human esophageal epithelial cells. Life Sci.75:21-34, 2004. https://www.ncbi.nlm.nih.gov/pubmed/15102519
  87. Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, and Umezawa A: Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis. Exp Cell Res.288:35-50, 2003. https://www.ncbi.nlm.nih.gov/pubmed/12878157
  88. Fujita M, Ichinose S, Kiyono T, Tsurumi T, and Omori A: Establishment of latrunculin-A resistance in HeLa cells by expression of R183A D184A mutant beta-actin. Oncogene.22:627-631, 2003. https://www.ncbi.nlm.nih.gov/pubmed/12555075
  89. Bruemmer D, Yin F, Liu J, Kiyono T, Fleck E, Van Herle A J, and Law R E: Rapamycin inhibits E2F-dependent expression of minichromosome maintenance proteins in vascular smooth muscle cells. Biochem Biophys Res Commun.303:251-258, 2003. https://www.ncbi.nlm.nih.gov/pubmed/12646195
  90. Bruemmer D, Yin F, Liu J, Kiyono T, Fleck E, Van Herle A J, and Law R E: Expression of minichromosome maintenance proteins in vascular smooth muscle cells is ERK/MAPK dependent. Exp Cell Res.290:28-37, 2003. https://www.ncbi.nlm.nih.gov/pubmed/14516785
  91. Bruemmer D, Yin F, Liu J, Kiyono T, Fleck E, Van Herle A, Graf K, and Law R E: Atorvastatin inhibits expression of minichromosome maintenance proteins in vascular smooth muscle cells. Eur J Pharmacol.462:15-23, 2003. https://www.ncbi.nlm.nih.gov/pubmed/12591091
  92. Bruemmer D, Yin F, Liu J, Berger J P, Kiyono T, Chen J, Fleck E, Van Herle A J, Forman B M, and Law R E: Peroxisome proliferator-activated receptor gamma inhibits expression of minichromosome maintenance proteins in vascular smooth muscle cells. Mol Endocrinol.17:1005-1018, 2003. https://www.ncbi.nlm.nih.gov/pubmed/12677008
  93. Kudoh A, Fujita M, Kiyono T, Kuzushima K, Sugaya Y, Izuta S, Nishiyama Y, and Tsurumi T: Reactivation of lytic replication from B cells latently infected with Epstein-Barr virus occurs with high S-phase cyclin-dependent kinase activity while inhibiting cellular DNA replication. J Virol.77:851-861, 2003. https://www.ncbi.nlm.nih.gov/pubmed/12502801
  94. Nagata K, Kawajiri A, Matsui S, Takagishi M, Shiromizu T, Saitoh N, Izawa I, Kiyono T, Itoh T J, Hotani H, and Inagaki M: Filament formation of MSF-A, a mammalian septin, in human mammary epithelial cells depends on interactions with microtubules. J Biol Chem.278:18538-18543, 2003. https://www.ncbi.nlm.nih.gov/pubmed/12626509
  95. Kyo S, Nakamura M, Kiyono T, Maida Y, Kanaya T, Tanaka M, Yatabe N, and Inoue M: Successful immortalization of endometrial glandular cells with normal structural and functional characteristics. Am J Pathol.163:2259-2269, 2003. https://www.ncbi.nlm.nih.gov/pubmed/14633600
  96. Kiyono T: [Human papillomaviruses]. Nihon Rinsho.61 Suppl 3:562-567, 2003. https://www.ncbi.nlm.nih.gov/pubmed/12718029
  97. Okamoto T, Aoyama T, Nakayama T, Nakamata T, Hosaka T, Nishijo K, Nakamura T, Kiyono T, and Toguchida J: Clonal heterogeneity in differentiation potential of immortalized human mesenchymal stem cells. Biochem Biophys Res Commun.295:354-361, 2002. https://www.ncbi.nlm.nih.gov/pubmed/12150956
  98. Nakamura H, Fukami H, Hayashi Y, Kiyono T, Nakatsugawa S, Hamaguchi M, and Ishizaki K: Establishment of immortal normal and ataxia telangiectasia fibroblast cell lines by introduction of the hTERT gene. J Radiat Res.43:167-174, 2002. https://www.ncbi.nlm.nih.gov/pubmed/12238331
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