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Home > Organization > Divisions and Independent Research Units > Group for Development of Molecular diagnostics and Individualized Therapy > Division of Epigenomics > Research Projects > Development of method for analysis of aberrant DNA methylation

Development of method for analysis of aberrant DNA methylation

An accurate analysis of DNA methylation enables us to unravel fundamental mechanisms of cancers and to make accurate diagnoses. There are two major categories of DNA methylation analysis. One is an analysis of individual genomic regions  and the other is genome-wide DNA methylation analysis .

We played a pioneering role in the development of genome-wide analysis of DNA methylation. In 1997, we developed methylation-sensitive representational difference analysis (MS-RDA) (Ushijima et al., 1997 , Ushijima and Yamashita, 2009 ). Subsequently, we applied the method to various cancers, and identified many genes with aberrant DNA methylation (Takai et al., 2001 , Kaneda et al., 2002 , Miyamoto et al., 2003 , Hagihara et al., 2004 , Abe et al., 2005 , Miyamoto et al., 2005 , Furuta et al., 2006 ). Recently, using our accumulated knowledge, we made a significant improvement on DNA methylation microarray analysis .

In addition to analysis of DNA methylation itself, analysis of the factor of alteration of DNA methylation is also important. We are developing such a method .


Fig. Methods of analysis of each region of genes

Methods for analysis of individual genomic regions can be categorized by their flexibility and quantitativity. We mainly use the methods in red line boxes.